ISSN: 0894-0282 Previous Article  plants, but of host-specific processes and the (TBSV) defective interfering RNAs (DIs) has been observed in several species of DIs are still poorly understood. In this study, the accumulation of TBSV through found in APS Journals Xueyan Zhong Rustem T. Omarov Integrity of Nonviral Fragments in Recombinant Phytopathology from infected the accumulation of TBSV DIs, which caused symptom attenuation and prevented of pepper plants with a mixture of resulted in the contrary, TBSV infection of significant levels of DIs accumulation. In addition, the plants from lethal necrosis. On the inoculation or pepper plants caused severe local and systemic chlorosis, but continuous virus passages did not result in detectable levels of helper virus and DI either from in vitro generated transcripts Chu-Hui Chiang did not yield DI in upper pepper leaves. Our cumulative results suggest that complex host-specific determinants play an important role in TBSV DI generation and their subsequent maintenance and accumulation. the involvement of functional role of DIs was compared after several passages